Qiang Du, MD
Research Assistant Professor
MD, Harbin Medical University, Harbin, China, 1983
The Wnt/beta-catenin pathway and inflammation play an important role in carcinogenesis, but the molecular mechanism is still to be elucidated. My research interest focuses on finding ways to determine human inducible nitric oxide (NO) synthase (hiNOS) as a target of Wnt/beta-catenin signaling and the role of NF-KappaB in the interaction of Wnt/beta-catenin and hiNOS/NO pathways in cancer cells. Nitric oxide, an important mediator of inflammation, and beta-catenin, a component of the Wnt-adenomatous polyposis coli signaling pathway, contribute to the development of cancer. We first found that beta-catenin up-regulates hiNOS, which suggest a novel mechanism by which the Wnt/beta-catenin signaling pathway may contribute to cancer by increasing NO production. Furthermore, since the hiNOS gene is also regulated by nuclear factor kappaB (NF-kappaB), we tested the hypothesis that Wnt/beta-catenin signaling regulates cytokine- or tumor necrosis factor alpha (TNFalpha)-induced hiNOS expression through interaction with NF-kappaB. Our in vitro and in vivo data show that beta-catenin signaling inversely correlates with cytokine-induced hiNOS and other NF-kappaB-dependent gene expression. These findings underscore the complex role of Wnt/beta-catenin, NF-kappaB, and iNOS signaling in the pathophysiology of inflammation-associated carcinogenesis. It is clear that hiNOS/NO is a critical regulator for development of cancer. The long term goal of my research is to acquire a molecular understanding of the signaling of hiNOS/NO on the regulation of carcinogenesis through Wnt/beta-catenin and NF-kappaB.
Peiyuan Li , Qiang Du , Zhongxian Cao , Zhong Guo , John Evankovich , Wei Yan , Ying Chang , Lifang Shao , Donna beer Stolz , Allan Tsung, David A Geller . Interferon-gamma induces autophagy with growth inhibition and cell death in human hepatocellular carcinoma (HCC) cells through interferon-regulatory factor-1 (IRF-1). Cancer Letter. 2012 Jan 28;314(2):213-22. Epub 2011 Oct 1. | View Publication
Qiang Du and David A. Geller Cross-regulation between Wnt and NF-kB Signaling
pathways. Immunapathological Diseases Therapeutics 2010 volume 1-Issum 3, p155-181. | View Publication
Qiang Du and David A. Geller. Chapter 8 (p. 269-300): Molecular Regulation of inducible Nitric Oxide Synthase. Nitric Oxide: Biology and Pathobiology (Second Edition), Edited by Louis J. Ignarro. Academic Press, 2010.
Qiang Du, Xinglu Zhang, Jon Cardionl, Zongxian Cao, Zhong Guo, Lifang Shao, and David A. Geller. Wnt/beta-catenin signaling regulates cytokine-induced hiNOS expression by inhibiting NF-kB pathway in cancer cells. Cancer Research, 2009; May 1 ; 69 (9) ; 3764-71. | View Publication
Qiang Du, Kyung Soo Park, Zhong Guo, Peijun He, Makoto Nagashima, Lifang Shao, Rohit Sahai, David A. Geller, S. Perwez Hussain. Regulation of human iNOS expression by Wnt/beta-catenin signaling. Cancer Research, 2006 Jul 15; 66(14): 7024-31. | View Publication