Our laboratory has a long-standing record of examining the role of innate immunity in the systemic response to injury and infection, with particular expertise in calcium-dependent mechanisms. Dr. Rosengart’s laboratory has focused upon the mechanisms involved in the inflammatory processes that define the response to injury in relevant sepsis and trauma models such as LPS-induced inflammation and organ dysfunction, cecal ligation and puncture (CLP) polymicrobial sepsis, warm hepatic ischemia/reperfusion (I/R) injury, and hemorrhagic shock. By employing diverse strategies such as in vivo siRNA to generate genetically engineered mice lacking key components of the calcium-sensitive CaMK cascade, high-speed depth resolved optical mapping of calcium transients, electron and fluorescent microscopy of subcellular organelle and protein trafficking, and study of the ramifications of calcium supplementation on inflammation and organ dysfunction, Dr. Rosengart has contributed to the understanding of calcium-dependent regulation and signaling during the inflammatory states of sepsis and injury. The translational perspective of his studies has provided mechanistic insight into the ramifications of the common practice of calcium supplementation in critical illness on the clinically relevant outcomes of organ failure and survival.
Persistence of Elevated Plasma CXCL8 Concentrations Following Red Blood Cell Transfusion in a Trauma Cohort. Lee JS, Sperry JL, Ochoa JB, Barclay D, Namas R, Vodovotz Y, Rosengart MR.Shock. 2012 Jan 27. | View Publication
Splenocyte Apoptosis and Autophagy is Mediated by Interferon Regulatory Factor-1 During Murine Endotoxemia.
Zhang L, Cardinal JS, Pan P, Rosborough BR, Chang Y, Yan W, Huang H, Billiar TR, Rosengart MR, Tsung A.Shock. 2012 Jan 18. | View Publication
Heme oxygenase-1-mediated autophagy protects against hepatocyte cell death and hepatic injury from infection/sepsis in mice. Carchman EH, Rao J, Loughran PA, Rosengart MR, Zuckerbraun BS.
Hepatology. 2011 Jun;53(6):2053-62. doi: 10.1002/hep.24324. Epub 2011 May 2. | View Publication
Calcium/calmodulin-dependent protein kinase (CaMK) Ialpha mediates the macrophage inflammatory response to sepsis. Zhang X, Guo L, Collage RD, Stripay JL, Tsung A, Lee JS, Rosengart MR.J Leukoc Biol. 2011 Aug;90(2):249-61. Epub 2011 Mar 3. | View Publication
Lipopolysaccaride induces autophagic signaling in macrophages via a TLR4, heme oxygenase-1 dependent pathway. Waltz P, Carchman EH, Young AC, Rao J, Rosengart MR, Kaczorowski D, Zuckerbraun BS.
Autophagy. 2011 Mar;7(3):315-20. | View Publication
Parenteral calcium for intensive care unit patients. Forsythe RM, Wessel CB, Billiar TR, Angus DC, Rosengart MR.
Cochrane Database Syst Rev. 2008 Oct 8;(4):CD006163. Review. | View Publication
Calcium/calmodulin-dependent protein kinase (CaMK) IV mediates nucleocytoplasmic shuttling and release of HMGB1 during lipopolysaccharide stimulation of macrophages.Zhang X, Wheeler D, Tang Y, Guo L, Shapiro RA, Ribar TJ, Means AR, Billiar TR, Angus DC, Rosengart MR. J Immunol. 2008 Oct 1;181(7):5015-23. | View Publication
HMGB1 release induced by liver ischemia involves Toll-like receptor 4 dependent reactive oxygen species production and calcium-mediated signaling. Tsung A, Klune JR, Zhang X, Jeyabalan G, Cao Z, Peng X, Stolz DB, Geller DA, Rosengart MR, Billiar TR. J Exp Med. 2007 Nov 26;204(12):2913-23. Epub 2007 Nov 5. | View Publication
Inverse association of plasma IL-13 and inflammatory chemokines with lung function impairment in stable COPD: a cross-sectional cohort study. Lee JS, Rosengart MR, Kondragunta V, Zhang Y, McMurray J, Branch RA, Choi AM, Sciurba FC. Respir Res. 2007 Sep 14;8:64. | View Publication