NW 607 MUH
The main focus of the laboratory is to understand the role of the immune system in lung pathophysiologies, such as pulmonary hypertension. The presence of immune cells in patients’ pulmonary lesions points to a role of the immune system in this disease. We are especially interested in understanding the role of endogenous innate immune mediators (i.e.HMGB1), and receptors (Toll- like receptors), as well as the interplay of innate immune cells such as Natural Killer cells, Neutrophils, and Macrophages. Using a variety of rodent disease models coupled with genetically modified mice and cell culture models we explore the role of the immune system in the pathogenesis of this disease. We further aim to learn lessons from cancer immunotherapies by applying their mechanisms to our lung-pathophysiologies.
At a more basic scientific level we try to get an in depth understanding of HMGB1’s interaction with toll-like receptors, its nuclear role, its travel through the three dimensional cell, and the purpose of HMGB1’s newly identified binding partners by us and our colleagues in the department of surgery.
Another interest of the lab is to advance our understanding of the immune system in inflammatory lung pathologies by collaborating with Dr. Carolyn Anderson in the dept. of radiology to image immune cells in our in vivo models using Positron Emission Tomography.
Pribis JP, Al-Abed Y, Yang H, Gero D, Xu H, Montenegro MF, Bauer EM, Kim S, Chavan SS, Cai C, Li T, Szoleczky P, Szabo C, Tracey KJ, Billiar TR: The HIV protease inhibitor saquinavir inhibits HMGB1 driven inflammation by targeting the interaction of cathepsin V with TLR4/MyD88.Mol Med. 2015 Sep 2. PMID: 26349060
Bauer, Eileen. Using the macrophage as a therapeutic tool - an inspirational session on “Jùshìxìbāo” the “macrophage, PVRI Chronicle, Volume 2, Issue 2. http://www.pvri.info/pvri-chronicle/vol-2/issue-2 - July 2015 - online journal only
Eileen M. Bauer, Chanthaphavong RS, Sodhi CP, Hackam DJ, Billiar TR, Philip M. Bauer. Genetic deletion of TLR4 on platelets attenuates experimental pulmonary hypertension. Circ Res. 2014 May 9;114(10):1596-600
Eileen M. Bauer, Han Zheng, Michael T. Lotze, Philip M. Bauer. Recombinant human interferon alpha 2b prevents and reverses experimental pulmonary hypertension. PLoS One. 2014 May 16;9(5):e96720
Bauer EM, Shapiro R, Billiar TR, Bauer PM. High Mobility Group Box-1 Inhibits Human Pulmonary Artery Endothelial Cell Migration via a TLR4 and IRF3-dependent Mechanism (s). J Biol Chem. 2013 Nov 12.
Eileen M. Bauer, Richard Shapiro, Han Zheng, Ferhaan Ahmad, David Ishizawar, Suzy A. Comhair, Serpil C. Erzurum, Timothy R. Billiar, and Philip M. Bauer. HMGB1 promotes experimental PH, Mol Med. 2013 Feb 8;18:1509-18.
Cruz JA; Bauer EM; Rodriguez AI; Gangopadhyay A; Zeineh NS; Wang Y; Shiva SS; Champion HC; Bauer PM. Endothelial dysfunction in the right ventricle promotes right heart failure in hypoxic Caveolin-1 -/-mice. Am J Physiol Heart Circ Physiol. 2012
Bauer PM, Bauer EM, Rogers NM, Yao M, Feijoo-Cuaresma M, Zuckerbraun BS, Calzada MJ, Isenberg JS. (2012) Activated CD47 promotes pulmonary arterial hypertension through targeting caveolin-1. Cardiovasc. Res .2012.
P. Bernal, K Leelavanichkul, E Bauer, R Cao, A Wilson, K Wasserloos, S Watkins, B Pitt and C. St. Croix. Nitric oxide mediated zinc release contributes to hypoxic regulation of pulmonary vascular tone. Circ Res. 102(12):1575-83. 2008
St.Croix CM, Bauer EM, Use of spectral fluorescence resonance energy transfer to detect nitric oxide-based signaling events in isolated perfused lung. Curr Protoc Cytom. Chapter 12:Unit12.13. 2008
Bernal PJ, Bauer EM, Cao R, Maniar S, Mosher M, Chen J, Wang QJ, Glorioso JC, Pitt BR, Watkins SC, St Croix CM. A Role for Zinc in Regulating Hypoxia-Induced Contractile Events in Pulmonary Endothelium, Am J Physiol Lung Cell Mol Physiol. 2011
Gambhir SS, Bauer E, Black ME, Liang Q, Kokoris MS, Barrio JR, Iyer M, Namavari M, Phelps ME, Herschman HR. A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography. PNAS U S A. 97(6):2785-90.2000